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beta-Actin Antibody

Catalog # Availability Size / Price Inquiry
APR15135G 100 μl / $495

beta-Actin Antibody

Brand

Leading Biology

Catalog Number

APR15135G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality beta-Actin antibody.

Molecular Weight

42kDa

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Hamster, Monkey

Clone

8H10D10

Isotype

IgG2b

GeneID

UniProt ID

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Form

Liquid. In PBS containing 0.02% sodium azide.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, ICC, E

Dilution

WB~~1/500 - 1/2000 IF~~1/200 - 1/1000 FC~~1/200 - 1/400 IHC~~1:200~~1000

Synonyms

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Images

Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.

Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.

Figure 3: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).

Specification

Quantity

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