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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > KRT5 Antibody (monoclonal) (M08)

KRT5 Antibody (monoclonal) (M08)

Catalog # Availability Size / Price Inquiry
APR17127G 100 μg / $495

KRT5 Antibody (monoclonal) (M08)

Brand

Leading Biology

Catalog Number

APR17127G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRT5 antibody (monoclonal) (M08).

Molecular Weight

62378 Da

Host

mouse

Species Reactivity

Human

Target

KRT5 (AAH24292, 1 a.a. ~ 590 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

1A12

Isotype

IgG2a Kappa

GeneID

UniProt ID

Summary

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Form

Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IP, E

Dilution

WB~~1:500~1000

Synonyms

Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5

Images

Western Blot analysis of KRT5 expression in transfected 293T cell line by KRT5 monoclonal antibody (M08), clone 1A12. Lane 1: KRT5 transfected lysate(62.4 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation of KRT5 transfected lysate using anti-KRT5 monoclonal antibody and Protein A Magnetic Bead (<a href="products_detail.asp?Catalog_id=U0007">U0007</a>), and immunoblotted with KRT5 MaxPab rabbit polyclonal antibody.

Detection limit for recombinant GST tagged KRT5 is 0.3 ng/ml as a capture antibody.

Specification

Quantity

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