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RAB5C Antibody

AMM07482G Product Datasheet Protocol

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AMM07482G		100 μl / $495

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Leading Biology Inc.

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RAB5C Antibody

Brand	Leading Biology	Catalog Number	AMM07482G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB5C antibody.
Molecular Weight	23483 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Host	Mouse
Species Reactivity	Human
Target	This RAB5C antibody is generated from a mouse immunized with a recombinant protein of human RAB5C.
Clone	1616CT314.65.31
Isotype	IgG2b,k
Symbol	RABL
GeneID	5878
UniProt ID	P51148
Function	Protein transport. Probably involved in vesicular traffic (By similarity).
Summary	Protein transport. Probably involved in vesicular traffic (By similarity).
Form	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, E
Dilution	FC~~1:25 WB~~1:2000
Synonyms	Ras-related protein Rab-5C, L1880, RAB5L, RAB5C, RABL
Images	Overlay histogram showing U-2OS cells stained with AMM07482G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM07482G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RAB5C Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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