Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > MKRN2 Antibody

MKRN2 Antibody

AMM06406G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
AMM06406G		100 μl / $495

Bulk Orders Inquiry

3 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

MKRN2 Antibody

Brand	Leading Biology	Catalog Number	AMM06406G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MKRN2 antibody.
Molecular Weight	46940 Da
Host	Mouse
Species Reactivity	Human
Target	This MKRN2 antibody is generated from a mouse immunized with recombinant protein.
Clone	1556CT631.230.55.49
Isotype	IgG1,k
Symbol	RNF62
GeneID	23609
UniProt ID	Q9H000
Function	E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Summary	E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Form	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, FC, WB, E
Dilution	IHC-P~~1:25 FC~~1:25 WB~~1:4000
Synonyms	Probable E3 ubiquitin-protein ligase makorin-2, 632-, RING finger protein 62, MKRN2, RNF62
Images	AMM06406G staining MKRN2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing K562 cells stained with AMM06406G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM06406G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-MKRN2 Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: RAB27B Antibody Next Product: FGF23 (Fibroblast Growth Factor 23) Anti...

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APG02467G	CCK4 / PTK7 Antibody (clone 4F9)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
2	Leading Biology	AMM04683G	GALT Antibody (clone 4C11)	50 μg	Monoclonal Antibodies				$545.00	Add Ask
3	Leading Biology	AMM01402G	Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
4	Leading Biology	APR08280G	LTA4H / LTA4 Antibody (clone 9G8)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
5	Leading Biology	AMM00172G	CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
6	Leading Biology	AMM05750G	CEBPA Antibody	100 μl	Monoclonal Antibodies				$545.00	Add Ask