> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > CAPN2 AntibodyBrand |
Leading Biology | Catalog Number |
APG02359G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CAPN2 antibody.
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Molecular Weight |
79995 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding
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Host |
Mouse
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Species Reactivity |
Human
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Immunogen |
1-400 aa
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Target |
This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.
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Clone |
1381CT669.7.66.71
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Isotype |
IgG2b
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Symbol |
CANPL2
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GeneID |
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UniProt ID |
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Function |
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).
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Summary |
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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Form |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, FC, IHC-P, E
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Dilution |
IHC-P~~1:25
FC~~1:100
WB~~1:1000
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Synonyms |
Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
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Images |
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, APG02359G) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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