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CAPN2 Antibody

Catalog # Availability Size / Price Inquiry
APG02359G 100 μl / $495

CAPN2 Antibody

Brand

Leading Biology

Catalog Number

APG02359G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAPN2 antibody.

Molecular Weight

79995 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding

Host

Mouse

Species Reactivity

Human

Immunogen

1-400 aa

Target

This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.

Clone

1381CT669.7.66.71

Isotype

IgG2b

Symbol

CANPL2

GeneID

UniProt ID

Function

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508).

Summary

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, IHC-P, E

Dilution

IHC-P~~1:25 FC~~1:100 WB~~1:1000

Synonyms

Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2

Images

Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, APG02359G) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody( APG02359G). APG02359G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Specification

Quantity

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