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UCHL1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR13915G 100 μl / $495

UCHL1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR13915G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UCHL1 antibody (C-term).

Molecular Weight

24824 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain

Host

Mouse

Species Reactivity

Human, Rat

Target

This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.

Clone

696CT2.1.4

Isotype

IgG1

GeneID

UniProt ID

Function

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Summary

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

IHC-P~~1:25 WB~~1:1000

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1

Images

Immunohistochemical analysis of paraffin-embedded H. brain section using UCHL1 Antibody (C-term)( AM2207B). AM2207B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. astroglioma section using UCHL1 Antibody (C-term)( AM2207B). AM2207B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

UCHL1 Antibody (C-term)(Cat. APR13915G) western blot analysis in 293T,NCI-H1299,rat PC-12 cell line lysates (35μg/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).

Specification

Quantity

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