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ALDH2 Antibody

Catalog # Availability Size / Price Inquiry
APG01744G 100 μl / $495

ALDH2 Antibody

Brand

Leading Biology

Catalog Number

APG01744G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALDH2 antibody.

Molecular Weight

56381 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion matrix.

Host

Mouse

Species Reactivity

Human

Target

This ALDH2 Monoclonal antibody is generated from mouse immunized with ALDH2 recombinant protein.

Clone

138CT22.3.8

Isotype

IgG1,Igκ

Symbol

ALDM

GeneID

UniProt ID

Summary

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, IF, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 IF~~1:10~50

Synonyms

Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM

Images

Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody( APG01744G). APG01744G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

ALDH2 Monoclonal Antibody (Cat. APG01744G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ALDH2 Monoclonal Antibody (Cat. APG01744G) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Specification

Quantity

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