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TBB5 Antibody

Catalog # Availability Size / Price Inquiry
APR11292G 100 μl / $495

TBB5 Antibody

Brand

Leading Biology

Catalog Number

APR11292G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TBB5 antibody.

Molecular Weight

49671 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton.

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Target

TBB5 recombinant protein is used to produce this monoclonal antibody.

Clone

87CT59.3.7

Isotype

IgG2b,Igκ

Symbol

TUBB5

GeneID

UniProt ID

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IHC-P, WB, IF, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 IF~~1:25

Synonyms

Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5

Images

Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody( APR11292G). APR11292G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody( APR11292G). APR11292G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. APR11292G). APR11292G was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor? 554 (red) conjugated Phalloidin (red).

Specification

Quantity

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