> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > GFAP AntibodyBrand |
Leading Biology | Catalog Number |
AMM05908G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GFAP antibody.
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Molecular Weight |
49880 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Note=Associated with intermediate filaments
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Host |
Mouse
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Species Reactivity |
Human
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Target |
This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
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Clone |
183CT3.1.5
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Isotype |
IgG2b,k
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GeneID |
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UniProt ID |
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Function |
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Summary |
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Form |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, IF, E
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Dilution |
WB~~1:100~500
IHC-P~~1:50~100
IF~~1:10~50
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Synonyms |
Glial fibrillary acidic protein, GFAP, GFAP
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Images |
All lanes : Anti-GFAP Antibody at 1:4000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
GFAP Antibody (Cat. AMM05908G) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
GFAP Monoclonal antibody (Cat. AMM05908G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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