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GFAP Antibody

Catalog # Availability Size / Price Inquiry
AMM05908G 100 μl / $495

GFAP Antibody

Brand

Leading Biology

Catalog Number

AMM05908G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GFAP antibody.

Molecular Weight

49880 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Note=Associated with intermediate filaments

Host

Mouse

Species Reactivity

Human

Target

This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.

Clone

183CT3.1.5

Isotype

IgG2b,k

GeneID

UniProt ID

Function

GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Summary

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, E

Dilution

WB~~1:100~500 IHC-P~~1:50~100 IF~~1:10~50

Synonyms

Glial fibrillary acidic protein, GFAP, GFAP

Images

All lanes : Anti-GFAP Antibody at 1:4000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

GFAP Antibody (Cat. AMM05908G) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).

GFAP Monoclonal antibody (Cat. AMM05908G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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