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Beclin 1 Antibody (Ascites)

APR06985G Product Datasheet Protocol

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APR06985G		0.1 ml / $495

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Leading Biology Inc.

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Beclin 1 Antibody (Ascites)

Brand	Leading Biology	Catalog Number	APR06985G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Beclin 1 antibody (Ascites).
Molecular Weight	51896 Da
Cellular Localization	Antigen Cellular Localization: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity)
Host	Mouse
Species Reactivity	Human
Target	This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Clone	15CT26
Isotype	IgG3λ
Symbol	GT197
GeneID	8678
UniProt ID	Q14457
Function	Plays a central role in autophagy (PubMed:23184933). Required for the abcission step in cytokinesis (PubMed:20208530). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Summary	Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).
Form	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	IF~~1:100 WB~~1:50~2000 IHC-P~~1:50~1:200
Synonyms	Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Images	Fluorescent image of U251 cells stained with APR06985G Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APR06985G Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. Western blot analysis of anti-BECN1 Mab (Cat. APR06985G) in HepG2, 293, Jurkat and Hela cell line lysates (35μg/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution). Western blot analysis of anti-BECN1 Mab (Cat. APR06985G) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).
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