> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > FE65 AntibodyBrand |
Leading Biology | Catalog Number |
APR11899G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality FE65 antibody.
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Molecular Weight |
77244 Da
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Cellular Localization |
Antigen Cellular Localization:
Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle Note=Colocalizes with TSHZ3 in axonal growth cone (By similarity) In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Colocalizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).
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Host |
Rabbit
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Species Reactivity |
Human
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Target |
A synthetic peptide corresponding to residues in human FE65 was used as an immunogen.
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Clone |
EPR3538
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Symbol |
FE65, RIR
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GeneID |
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UniProt ID |
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Function |
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro- apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
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Summary |
Amyloid beta A4 precursor protein-binding family B member 1 (FE65) is a member of a family of multidomain adaptor proteins (FE65, FE65L1, and FE65L2) that form multiprotein complexes with a range of functions. FE65 is highly expressed in the brain and it has three protein-protein interaction domains: a WW domain and two PID/PTB domains. Through the PTB2 domain, FE65 interacts with the Alzheimer?s disease amyloid precursor protein (APP) intracellular domain (AICD) and can alter APP processing. The WW domain is responsible for the interaction with various proteins, which in turn interacts with the cytoskeleton. The second PID/PTB domain of FE65 binds to the transcription factor, CP2/LSF/LBP1 protein (1, 2). FE65 also plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis (3).
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Form |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC
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Dilution |
WB~~1:2000
IHC~~1:50~100
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Synonyms |
Amyloid beta A4 precursor protein-binding family B member 1, Protein Fe65, APBB1, FE65, RIR
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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