> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Caspase-3 Antibody (Pro)Brand |
Leading Biology | Catalog Number |
APG02400G |
Product Type |
Monoclonal Antibodies | Field of Research |
|
Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Caspase-3 antibody (Pro).
|
||
Molecular Weight |
31608 Da
|
||
Cellular Localization |
Antigen Cellular Localization:
Cytoplasm.
|
||
Host |
Rabbit
|
||
Species Reactivity |
Human
|
||
Target |
A synthetic peptide correscponding to N-terminal residues of Human Pro-Caspase-3 was used as immunogen. The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3.
|
||
Clone |
E61
|
||
Symbol |
CPP32
|
||
GeneID |
|||
UniProt ID |
|||
Function |
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
|
||
Summary |
Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
|
||
Form |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
||
Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
||
Applications |
WB, IHC, FC, IF
|
||
Dilution |
WB~~1:1000
IHC~~1:500
FC~~1:50
|
||
Synonyms |
Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32
|
||
Images |
|
||
Specification |
|||
Quantity |
|
||
| Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
| 1 | Leading Biology | APG02467G | CCK4 / PTK7 Antibody (clone 4F9) | 50 μl | Monoclonal Antibodies |
|
$495.00 | Add Ask | ||
| 2 | Leading Biology | AMM04683G | GALT Antibody (clone 4C11) | 50 μg | Monoclonal Antibodies |
|
$545.00 | Add Ask | ||
| 3 | Leading Biology | AMM01402G | Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide | 50 ug | Monoclonal Antibodies |
|
$395.00 | Add Ask | ||
| 4 | Leading Biology | APR08280G | LTA4H / LTA4 Antibody (clone 9G8) | 50 μl | Monoclonal Antibodies |
|
$495.00 | Add Ask | ||
| 5 | Leading Biology | AMM00172G | CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody - With BSA and Azide | 50 ug | Monoclonal Antibodies |
|
$395.00 | Add Ask | ||
| 6 | Leading Biology | AMM05750G | CEBPA Antibody | 100 μl | Monoclonal Antibodies |
|
$545.00 | Add Ask |
Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
You have 0 item in your cart
You have 0 item in your inquiry list
