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BRAF Antibody

Catalog # Availability Size / Price Inquiry
AMM02626G 100 μl / $545

BRAF Antibody

Brand

Leading Biology

Catalog Number

AMM02626G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BRAF Antibody.

Molecular Weight

84437 Da

Cellular Localization

Antigen Cellular Localization: Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

Host

Mouse

Species Reactivity

Human

Clone

1H12F1; 1H12G10; 1F12F11C9

Isotype

IgG1

Symbol

BRAF1, RAFB1

GeneID

UniProt ID

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC, E

Dilution

WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 IF~~1:200~1000.

Images

Figure 1: Western blot analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).

Figure 2: Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).

Figure 3: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB staining.

Specification

Quantity

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