> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > RAD18 AntibodyBrand |
Leading Biology | Catalog Number |
AMM03063G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RAD18 Antibody.
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Molecular Weight |
56.2kDa
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Cellular Localization |
Antigen Cellular Localization:
Nucleus.
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Host |
Mouse
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Species Reactivity |
Human
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Clone |
2G8D12
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Isotype |
IgG1
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Symbol |
RNF73
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GeneID |
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UniProt ID |
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Function |
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC, FC, ICC, E
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Dilution |
E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
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Images |
Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);
Figure 2:Western blot analysis using RAD18 mAb against human RAD18 (AA: 332-430) recombinant protein. (Expected MW is 37.3 kDa)
Figure 3:Western blot analysis using RAD18 mAb against HEK293 (1) and RAD18 (AA: 332-430)-hIgGFc transfected HEK293 (2) cell lysate. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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