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CRTC3 Antibody

AMM02823G Product Datasheet Protocol

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AMM02823G		100 μl / $545

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Leading Biology Inc.

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CRTC3 Antibody

Brand	Leading Biology	Catalog Number	AMM02823G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CRTC3 Antibody.
Molecular Weight	68kDa
Cellular Localization	Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Appears to be mainly nuclear
Host	Mouse
Species Reactivity	Human, Monkey
Clone	5G9
Isotype	IgG1
Symbol	TORC3
GeneID	64784
UniProt ID	Q6UUV7
Function	Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC, ICC, E
Dilution	E~~1/10000 WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 IF~~1/200 - 1/1000
Images	Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng); Figure 1: Western blot analysis using CRTC3 mouse mAb against Hela (1), Jurkat (2), Cos7 (3) and MCF-7 (4) cell lysate. Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer (left) and ovarian cancer (right) using CRTC3 mouse mAb with DAB staining.
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