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F8 Antibody (monoclonal) (M03)

Catalog # Availability Size / Price Inquiry
AMM03510G 100 μg / $495

F8 Antibody (monoclonal) (M03)

Brand

Leading Biology

Catalog Number

AMM03510G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality F8 Antibody (monoclonal) (M03).

Molecular Weight

267009 Da

Host

mouse

Species Reactivity

Human

Target

F8 (NP_000123, 213 a.a. ~ 312 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

1E9

Isotype

IgG2b Kappa

GeneID

UniProt ID

Summary

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

E

Images

Detection limit for recombinant GST tagged F8 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between CALR and F8. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-F8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Specification

Quantity

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