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RASSF2 Antibody

AMM02545G Product Datasheet Protocol

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AMM02545G		100 μl / $495

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Leading Biology Inc.

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RASSF2 Antibody

Brand	Leading Biology	Catalog Number	AMM02545G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RASSF2 Antibody.
Molecular Weight	37790 Da
Cellular Localization	Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1
Host	Mouse
Species Reactivity	Human
Target	This RASSF2 antibody is generated from a mouse immunized with a recombinant protein between 1-326 amino acids from human RASSF2.
Clone	1509CT269.10.9.29
Isotype	IgG2a,κ
Symbol	KIAA0168
GeneID	9770
UniProt ID	P50749
Function	Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Summary	Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, E
Dilution	FC~~1:25 WB~~1:2000
Images	Overlay histogram showing Raji cells stained with AMM02545G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02545G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-RASSF2 Antibody at 1:2000 dilution + Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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