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RAB3B Antibody

AMM02529G Product Datasheet Protocol

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AMM02529G		100 μl / $495

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Leading Biology Inc.

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RAB3B Antibody

Brand	Leading Biology	Catalog Number	AMM02529G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB3B Antibody.
Molecular Weight	24758 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
Host	Mouse
Species Reactivity	Human, Mouse, Rat
Target	This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B.
Clone	1543CT354.60.92
Isotype	IgG1,k
GeneID	5865
UniProt ID	P20337
Function	Protein transport. Probably involved in vesicular traffic (By similarity).
Summary	Protein transport. Probably involved in vesicular traffic (By similarity).
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, IHC-P, WB, E
Dilution	FC~~1:25 IHC-P~~1:25 WB~~1:2000
Images	Overlay histogram showing HepG2 cells stained with AMM02529G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02529G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. AMM02529G staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-RAB3B Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lane 3: SW480 whole cell lysate Lane 4: human brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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