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RAB3A Antibody

AMM02525G Product Datasheet Protocol

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AMM02525G		100 μl / $495

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Leading Biology Inc.

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RAB3A Antibody

Brand	Leading Biology	Catalog Number	AMM02525G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB3A Antibody.
Molecular Weight	24984 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
Host	Mouse
Species Reactivity	Human, Mouse, Rat
Target	This RAB3A antibody is generated from a mouse immunized with a recombinant protein.
Clone	1531CT562.14.57
Isotype	IgG1,k
GeneID	5864
UniProt ID	P20336
Function	Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Summary	Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, FC, WB, E
Dilution	IF~~1:25 FC~~1:25 WB~~1:500
Images	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized PC-12 (rat adrenal phaeochromocytoma cell line) cells labeling Pdx1 with AMM02525G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on PC-12 cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). Overlay histogram showing PC-12 cells stained with AMM02525G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02525G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RAB3A Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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