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CD74 Antibody

Catalog # Availability Size / Price Inquiry
AMM02483G 100 μl / $495

CD74 Antibody

Brand

Leading Biology

Catalog Number

AMM02483G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD74 Antibody.

Molecular Weight

33516 Da

Cellular Localization

Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane

Host

Mouse

Species Reactivity

Human

Target

This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-232 amino acids from human.

Clone

1267CT820.116.140.154

Isotype

IgG2b,κ

Symbol

DHLAG

GeneID

UniProt ID

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

Summary

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

FC~~1:25 WB~~1:1000

Images

Flow cytometric analysis of Raji cells using CD74(green, AMM02483G) compared to an isotype control of mouse IgG2b(blue). AMM02483G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from Daudi, Raji cell line (from left to right), using CD74 Antibody(Cat. AMM02483G). AMM02483G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Specification

Quantity

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