> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > CD74 AntibodyBrand |
Leading Biology | Catalog Number |
AMM02483G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CD74 Antibody.
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Molecular Weight |
33516 Da
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Cellular Localization |
Antigen Cellular Localization:
Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane
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Host |
Mouse
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Species Reactivity |
Human
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Target |
This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-232 amino acids from human.
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Clone |
1267CT820.116.140.154
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Isotype |
IgG2b,κ
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Symbol |
DHLAG
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GeneID |
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UniProt ID |
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Function |
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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Summary |
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, FC, E
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Dilution |
FC~~1:25
WB~~1:1000
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Images |
Flow cytometric analysis of Raji cells using CD74(green, AMM02483G) compared to an isotype control of mouse IgG2b(blue). AMM02483G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Daudi, Raji cell line (from left to right), using CD74 Antibody(Cat. AMM02483G). AMM02483G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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