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CD73(NT5E) Antibody (C-term)

AMM02468G Product Datasheet Protocol

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AMM02468G		100μl / $495

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Leading Biology Inc.

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CD73(NT5E) Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM02468G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD73(NT5E) Antibody (C-term).
Molecular Weight	63368 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
Host	Mouse
Species Reactivity	Human
Target	Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody.
Clone	974CT7.5.3
Isotype	IgG1
Symbol	NT5, NTE
GeneID	4907
UniProt ID	P21589
Function	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Summary	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, E
Dilution	IHC-P~~1:25 WB~~1:5000
Images	AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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