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EGFR Antibody (C-term)

AMM02448G Product Datasheet Protocol

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AMM02448G		100 μl / $495

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Leading Biology Inc.

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EGFR Antibody (C-term)

Brand	Leading Biology	Catalog Number	AMM02448G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EGFR Antibody (C-term).
Molecular Weight	134277 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Host	Mouse
Species Reactivity	Human
Immunogen	1163-1191 aa
Target	This EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR.
Clone	688CT33.1.3
Isotype	IgG1
Symbol	ERBB, ERBB1, HER1
GeneID	1956
UniProt ID	P00533
Function	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Summary	The protein encoded by this gene is a transmembraneglycoprotein that is a member of the protein kinase superfamily.This protein is a receptor for members of the epidermal growthfactor family. EGFR is a cell surface protein that binds toepidermal growth factor. Binding of the protein to a ligand inducesreceptor dimerization and tyrosine autophosphorylation and leads tocell proliferation. Mutations in this gene are associated with lungcancer. Multiple alternatively spliced transcript variants thatencode different protein isoforms have been found for this gene.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, E
Dilution	WB~~1:1000 IHC-P~~1:25
Images	All lanes : Anti-EGFR Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term)( AMM02448G). AMM02448G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term)( AMM02448G). AMM02448G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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