• Account
  • Account
    Inquiries
    0

    Inquired items

    0 items total Subtotal:$ 0
  • 会员中心
    Cart

    Added items

    0items total Subtotal:$ 0

All Departments

FGFR4 Antibody (Ascites)

Catalog # Availability Size / Price Inquiry
AMM02495G 0.1 ml / $495

FGFR4 Antibody (Ascites)

Brand

Leading Biology

Catalog Number

AMM02495G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality FGFR4 Antibody (Ascites).

Molecular Weight

87954 Da

Cellular Localization

Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Note=Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane Isoform 3: Cytoplasm

Host

Mouse

Species Reactivity

Human

Target

Purified recombinant FGFR4 fusion protein was used to produced this monoclonal antibody.

Clone

53CT32.19.3

Isotype

IgG1 κ

Symbol

JTK2, TKF

GeneID

UniProt ID

Function

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

Summary

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:2000

Images

Western blot analysis of anti-FGFR4 Monoclonal Antibody (Cat.AMM02495G) by FGFR4 recombinant protein(Fragment 42KD). FGFR4 protein (arrow) was detected using the ascites Mab.

Western blot analysis of FGFR4 (arrow) using mouse monoclonal FGFR4 antibody(Ascites) (Cat.AMM02495G). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies) (1:2000)

Specification

Quantity

Related Products

Select Brand Catalog No. Product Name Pack Size Type Field of Research Specification Quantity Price(USD)
1 Leading Biology AMM07977G SPRY1 / Sprouty 1 Antibody (clone 3H4) 50 μg Monoclonal Antibodies
$545.00 Add Ask
2 Leading Biology AMM00137G CD79a (B-Cell Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
3 Leading Biology APG02467G CCK4 / PTK7 Antibody (clone 4F9) 50 μl Monoclonal Antibodies
$495.00 Add Ask
4 Leading Biology AMM05365G DC-SIGN Antibody [5D7] 0.1 mg Monoclonal Antibodies
$695.00 Add Ask
5 Leading Biology AMM01402G Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
6 Leading Biology APR08280G LTA4H / LTA4 Antibody (clone 9G8) 50 μl Monoclonal Antibodies
$495.00 Add Ask
Contact Us

This item has successfully been added to your cart!

You have 0 item in your cart

Your inquiry has successfully been added to your list!

You have 0 item in your inquiry list