> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > MLH1 Antibody (Center) (Ascites)Brand |
Leading Biology | Catalog Number |
AMM02440G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MLH1 Antibody (Center) (Ascites).
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Molecular Weight |
84601 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus
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Host |
Mouse
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Species Reactivity |
Human
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Immunogen |
458-489 aa
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Target |
This MLH1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 458-489 amino acids from the Central region of human MLH1.
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Clone |
628CT4.6.1
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Isotype |
IgG1
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Symbol |
COCA2
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GeneID |
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UniProt ID |
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Function |
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
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Summary |
This gene was identified as a locus frequently mutated inhereditary nonpolyposis colon cancer (HNPCC). It is a human homologof the E. coli DNA mismatch repair gene mutL, consistent with thecharacteristic alterations in microsatellite sequences(RER+phenotype) found in HNPCC. Alternative splicing results inmultiple transcript variants encoding distinct isoforms. Additionaltranscript variants have been described, but their full-lengthnatures have not been determined.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, E
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Dilution |
WB~~1:100~400
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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