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ALPP Antibody (N-term)(Ascites)

AMM02400G Product Datasheet Protocol

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AMM02400G		0.1 ml / $495

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Leading Biology Inc.

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ALPP Antibody (N-term)(Ascites)

Brand	Leading Biology	Catalog Number	AMM02400G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALPP Antibody (N-term)(Ascites).
Molecular Weight	57954 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
Host	Mouse
Species Reactivity	Human
Immunogen	56-83 aa
Target	This ALPP antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the N-terminal region of human ALPP.
Clone	421CT8.1.1
Isotype	IgG1
Symbol	PLAP
GeneID	250
UniProt ID	P05187
Summary	There are at least four distinct but related alkalinephosphatases: intestinal, placental, placental-like, andliver/bone/kidney (tissue non-specific). The first three arelocated together on chromosome 2 while the tissue non-specific formis located on chromosome 1. The product of this gene is a membranebound glycosylated enzyme, also referred to as the heat stableform, that is expressed primarily in the placenta although it isclosely related to the intestinal form of the enzyme as well as tothe placental-like form. The coding sequence for this form ofalkaline phosphatase is unique in that the 3' untranslated regioncontains multiple copies of an Alu family repeat. In addition, thisgene is polymorphic and three common alleles (type 1, type 2 andtype 3) for this form of alkaline phosphatase have been wellcharacterized.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, E
Dilution	IF~~1:100 WB~~1:500~3200
Images	Fluorescent confocal image of HeLa cells stained with ALPP (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AMM02400G ALPP (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALPP immunoreactivity is localized to the cytoplasm of HeLa cells. ALPP Antibody (N-term) (Cat. AMM02400G) western blot analysis in mouse placenta tissue lysates (35μg/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).
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