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LGR5/GPR49 Antibody

AMM02389G Product Datasheet Protocol

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AMM02389G		100 μl / $495

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Leading Biology Inc.

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LGR5/GPR49 Antibody

Brand	Leading Biology	Catalog Number	AMM02389G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LGR5/GPR49 Antibody.
Molecular Weight	99998 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
Host	Mouse
Species Reactivity	Human
Immunogen	689-719 aa
Target	This LGR5/GPR49 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5/GPR49.
Clone	349CT16.1.3.1
Isotype	IgG1
Symbol	GPR49, GPR67
GeneID	8549
UniProt ID	O75473
Function	Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Summary	Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, E
Dilution	FC~~1:25 WB~~1：2000
Images	Overlay histogram showing Hela cells stained with AMM02389G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02389G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western blot analysis of lysates from K562 cell line (from left to right), using LGR5/GPR49 Antibody (Center)(AMM02389G). AMM02389G was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
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