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Location: Home > Tool Enzymes, Molecular Markers > DNA Polymerase > Hot start CH-Taq DNA Polymerase

Hot start CH-Taq DNA Polymerase

Catalog # Availability Size / Price Inquiry
MQ001-01 50 μL / $280
MQ001-02 4 x 50 μL / $1000

Hot start CH-Taq DNA Polymerase

Brand

Leading Biology

Catalog Number

MQ001

Product Type

DNA Polymerase

Field of Research

Product Overview

This product is a recombinant Taq DNA polymerase modified by chemical groups, suitable for Hot Start PCR. At room temperature, the activity of Taq DNA polymerase was blocked by chemical groups, which inhibited the non-specific amplification caused by non-specific annealing of template primers or primer dimers. When the amplification reaction system was heated to 95°C and heated for 10min, the chemical groups were detached from the polymerase and the polymerase activity was completely recovered.

Activity

One unit is the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.

Form

Liquid

Concentration

5 U/μL

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 24 months at -20°C. Avoid repeated freeze-thaw cycles.

Storage Buffer

Tris-HCl (pH8. 0) 20 mM KCl 100 mM EDTA 0. 1 mM DTT 1 Tween 20 0. 5% Nonidet P-40 0. 5% Glycerol 50%

Applications

PCR, hot start PCR, qPCR, genome PCR, TA cloning, primer extension, DNA markers.

Images

Specification

Quantity

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