> Tool Enzymes, Molecular Markers > Protein Modification Enzymes > Protease > Recombinant TEV proteaseBrand |
Leading Biology | Catalog Number |
EH0001E1 |
Product Type |
Protease | Field of Research |
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Expression Host |
E.coli
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Tags |
C-His
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Molecular Weight |
27KD
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Activity |
20U/μl
One unit of TEV cleaves more than 90% of 3 µg control substrate in 12 h at 30°C.
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Form |
Liquid |
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Concentration |
Greater than 95% by SDS-PAGE
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Storage & Stability |
-70°C
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Storage Buffer |
0.2 μm filtered 25 mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5 mM EDTA, 1mM DTT 50% glycerol
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Product Description |
TEV Protease is form of Tobacco Etch Virus (TEV) protease that is highly site-specific, stable, and active. TEV Protease recognizes the seven-amino-acid sequence E-N-L-Y-F-Q-S/G and cleaves between Q and S/G with high specificity. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature (4 - 30°C) and pH (pH 6.0-8.5). TEV Protease is purified from E. coli by affinity chromatography using the C-terminus polyhistidine tag. Following digestion, TEV Protease is easily removed from the cleavage reaction by Ni+ affinity chromatography.
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Synonyms |
rTEV, TEV, P1 protease
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Images |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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