Introduction

Western blot is an important technique that frequently used in molecular biology. It could let the researchers able to look at a single protein or a subset of proteins via a high sensitivity antibody binding. Western blot could be used to follow protein phosphorylation or identify known and unknown proteins in complexes.

There are three steps in Western blot in order to accomplish the tasks above: 

(1). The proteins, cell lysates or tissue lysates were run on SDS-polyacrylamide gels, after this step, the proteins were separated by size. 

(2). The fractionated proteins, polypeptides or fragments were transferred onto a PVDF membrane, and (3). Probed with a primary-secondary antibody pair to visualize.

Procedures:

1. Extraction of total proteins from tissues/cells.

2. Quantitation of total protein using sensitive, specific and accurate protein assay.

3. Fractionation of protein through large SDS-polyacrylamide gel electrophoresis.

4. Transfer protein onto PVDF membranes.

5. Immunostaining blots with antibodies.

6. Scanning protein bands on X-ray films.

7. Analysis of immunostaining data.

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

· Vigorous quality control system to ensure the required quality and reproducibility

· Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

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