Introduction

The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. In this assay, the light could be generated by one of a family of enzymes known as luciferases, and another essential tool in this study is the reporter gene, as they are designed to function as surrogates for genes involved in key cellular process and disease. A good reporter gene includes an easily measurable phenotype, low background activity in the cell under investigation, a large dynamic range, and high reliability and sensitivity. There are a number of genes that could meet these requirements, including members of the family of luciferases, β-galactosidase (β-Gal), chloramphenicol acetyltransferase (CAT), etc.

Typically, the reporter gene is cloned with a DNA sequence of interest into an expression vector that is then transferred into cells. After transfer, the cells are assayed for the presence of the reporter by directly measuring the reporter protein itself or enzymatic activity of the reporter protein. 

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