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Location: Home > Custom Services > Immunology Services > Immunohistochemistry Test

Immunohistochemistry Test

Date: 2018-01-25 Author: Leading Biology Click: 1733

Introduction

Immunohistochemistry (IHC) is a technique used to determine the presence and level of specific cellular proteins. IHC measures protein expression using specially labeled antibodies that can bind to the proteins of interest. The antibody is mixed with the cellular components of the tumor. After a set amount of time, the mixture is rinsed and only those antibodies attached to their protein targets will remain. The presence of the antibodies can be detected by viewing the sample under a microscope because areas containing bound antibodies will appear a different color than areas lacking antibodies. Samples with more protein will bind more antibody and therefore appear darker. This allows the test to reveal not only whether a protein is present but also the relative amount of the protein. Test results are based on the strength of the staining and the percent of cells stained.


Immunohistochemistry


Procedures:

1. Tissue Fixation
2. Antigen Retrieval
3. Staining/Add primary antibody
4. Add secondary antibody
5. Add chromogen
6. Counterstain

7. Detection


Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
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By Phone: 1-661-524(LBI)-0262 (USA)


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