Introduction
Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC) to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. IF allows researchers to evaluate whether cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, immunofluorescence also allows researchers to determine which subcellular compartments are expressing the antigen. IF can be used on cultured cell lines, tissue sections, or individual cells.
There are two different immunofluorescence assays: indirect immunofluorescence assay and direct immunofluorescence assay. For indirect immunofluorescence assay, the protocol mainly includes tissue or cell treparation, tissue or cell fixation, serum blocking, primary antibody incubation, marked second antibody incubation, staining, result judgment and imaging. For direct immunofluorescence assay, there are only marked primary antibody been incubated without the second antibody and other steps are the same.
Fig. 1 Indirect Immunofluorescence Assay
Why Leading Biology
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.
• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Contact Information
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
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