• Account
  • Account
    Inquiries
    0

    Inquired items

    0items total Subtotal:$ 0
  • Cart
    Cart

    Inquired items

    items total Subtotal:$ 0

All Departments

Location: Home > Custom Services > Immunology Services > Immunofluorescence Technique

Immunofluorescence Technique

Date: 2018-01-25 Author: Leading Biology Click: 1623

Introduction

Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC) to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. IF allows researchers to evaluate whether cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, immunofluorescence also allows researchers to determine which subcellular compartments are expressing the antigen. IF can be used on cultured cell lines, tissue sections, or individual cells.


There are two different immunofluorescence assays: indirect immunofluorescence assay and direct immunofluorescence assay. For indirect immunofluorescence assay, the protocol mainly includes tissue or cell treparation, tissue or cell fixation, serum blocking, primary antibody incubation, marked second antibody incubation, staining, result judgment and imaging. For direct immunofluorescence assay, there are only marked primary antibody been incubated without the second antibody and other steps are the same.



Indirect Immunofluorescence Assay


Fig. 1 Indirect Immunofluorescence Assay


Direct Immunofluorescence Assay

Fig. 2 Direct Immunofluorescence Assay



Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

 

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)


Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * captcha
No Headline Click Author Date
1 Genome Editing: Which Should I Choose, TALEN or CRISPR? 1609 Leading Biology 2020-01-03
2 TALEN - Transcription activator-like effector nuclease 2120 Leading Biology 2020-01-02
3 Microorganisms Gene Modification Services 1347 Leading Biology 2019-12-30
4 CRISPR-CAS Technology 1705 Leading Biology 2019-12-30
5 Transgenic Plants Construction 1644 Leading Biology 2019-12-30
6 In Situ Hybridization 2484 Leading Biology 2018-01-26