Construction And Packaging of Lentiviral Vectors _Molecular Biology Services_Custom Services

Introduction

Lentiviruses are a subset of retroviruses and are derived from the human immunodeficiency virus (HIV-1) with most of the viral genes removed, only contain the Long terminal repeats (LTRs) and the packaging sequence (Psi-sequence), thus they have the ability to transduce both dividing and non-dividing cells without significant immune responses. They also enable long-term transgene expression by integrating stably into the host genome.

To obtain a lentiviral vector, a target gene is cloned into a vector sequence which is flanked by LTRs and the Psi-sequence. The LTRs are necessary to integrate the therapeutic gene into the genome of the target cell, and the Psi-sequence acts as a signal sequence and is necessary for packaging RNA with the target gene in virions.

Construction And Packaging of Lentiviral Vectors

Procedures:
1. Construction of a lentivirus overexpressing plasmid vector.
2. Interference of lentivirus in the construction of plasmid vector.

3. Packaging and concentrated purification of lentiviral vectors.

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