Construction And Packaging of Adenovirus Vectors_Molecular Biology Services_Custom Services

Introduction

The adenovirus genome is a linear, 36-Kb double-stranded DNA (dsDNA) molecule containing multiple, heavily spliced transcripts. At either end of the genome are inverted terminal repeats (ITRs). Genes are divided into early (E1-E4) and late (L1-L5) transcripts. As a cell expression vectors, adenoviral vectors have a number of distinct advantages: its genome is relatively easy to manipulate by recombinant DNA techniques, and the adenovirus vectors are relatively stable, can grow to high titers, and can transduce a variety of cell types in cell culture in vivo.

Vectors can be designed that are either relocation competent or replication defective, and in the latter case, they are highly efficient at delivering and expressing genes in mammalian cells without killing the cells.

Construction And Packaging of Adenovirus Vectors

Procedures:
1. Cloning a Gene of Interest into Shuttle Vectors.
2. Transfer the expression cassette into the Adenovirus Vector.
3. Producing first generation, Low-titer Virus Stock.
4. Virus Amplification and Concentration.

5. Titration.

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