Southern Blot Service (non-radioactive labeled)_Molecular Biology Services_Custom Services

Introduction

Southern blot is a widely used technique in molecular biology for the detection of a specific DNA sequence in samples. It’s frequently used to detect the genome integrity and mutation.

In Southern bolt, the DNA will be extracted from testing samples followed by UV quantitation and electrophoresis analysis. After restriction digest with enzymes, DNA samples were applied to an agarose gel, then the fragments of DNA were separated by electrophoresis according to size. The gel then where transferred and UV cross-linked to a membrane. The DNA fragments on the membrane were then hybridized by a Chemiluminescent labeled probe. After hybridization, the labeled fragments will be visualized by X-ray.

 Southern blot Service (non-radioactive labeled)

Procedures:

1. Isolation DNA from samples.
2. Fractionation of DNA through denatured agarose gels.
3. Transfer DNA onto supercharged membranes.
4. Cross-linked DNA by UV light.
5. Hybridized membrane with labeled probes.

6. Visualization of the labeled DNA on X-ray film.

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

· Vigorous quality control system to ensure the required quality and reproducibility

· Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)

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