PCR/Q-PCR/RT-PCR Service_Molecular Biology Services_Custom Services

Introduction

Polymerase Chain Reaction is a widely used technique used to make millions or billions of copies of a particular DNA region. The PCR could be used to sequence DNA fragments, and further diagnose diseases, identify bacteria and viruses, etc.

Fig. 1 Reverse transcription polymerase chain reaction (RT-PCR)

Reverse transcription polymerase chain reaction (RT-PCR) is a PCR that is designed to detect and measure RNA. Compare to traditional PCR, RT-PCR first taking RNA and converting the RNA strand into its DNA complement. The new complementary DNA containing the reversed transcription will then be amplified.

Fig. 2 Quantitative Real-Time PCR

Quantitative PCR (qPCR) is used to detect, characterize and quantify nucleic acids. In contrast to traditional PCR end-point detection, qPCR combines PCR amplification and detection into a single step, which means it collects data as the reaction is proceeding. This eliminates using gel electrophoresis to detect the samples, and make the PCR to be truly quantitative. qPCR provides an accurate as well as fast and sensitive means to quantify the products.

Procedures:
1. RNA extraction from samples.
2. RNA quantitation and quality assessment using a spectrophotometer, and gel electrophoresis.
3. Design qPCR primers.
4. Set up and run the real-time RT-qPCR reactions.
5. Data analysis.

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

· Vigorous quality control system to ensure the required quality and reproducibility

· Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)

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