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Location: Home > Custom Services > Molecular Biology Services > Nucleic acid extraction

Nucleic acid extraction

Date: 2009-05-17 Author: Leading Biology Click: 5750

Introduction

Nucleic acid extraction plays a vital role in molecular biology since the quality of nucleic acids can affect the performance of downstream reactions, high quality and high yield of nucleic acids are necessary. Nucleic acid extraction including DNA isolation and RNA isolation.

DNA Isolation


There are four steps to extract DNA from cells or tissues.
1. Break the cell membranes open to expose the DNA (cell lysis)
2. The cell lysates are treated with a concentrated salt solution to make debris clump together.
3. Centrifuge the solution to separate the clumped cellular debris from the nucleic acid.
4. DNA could be isolated by adding ethanol, isopropanol, RNase or even use the minicolumn.


DNA Isolation

RNA Isolation



RNA isolation is similar to DNA isolation, only the last step is different. Instead of adding RNase into the precipitate, DNase was added, after purification, isolated RNA molecules were collected.



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