Introduction
Nucleic acid extraction plays a vital role in molecular biology since the quality of nucleic acids can affect the performance of downstream reactions, high quality and high yield of nucleic acids are necessary. Nucleic acid extraction including DNA isolation and RNA isolation.
DNA Isolation
There are four steps to extract DNA from cells or tissues.
1. Break the cell membranes open to expose the DNA (cell lysis)
2. The cell lysates are treated with a concentrated salt solution to make debris clump together.
3. Centrifuge the solution to separate the clumped cellular debris from the nucleic acid.
4. DNA could be isolated by adding ethanol, isopropanol, RNase or even use the minicolumn.
RNA isolation is similar to DNA isolation, only the last step is different. Instead of adding RNase into the precipitate, DNase was added, after purification, isolated RNA molecules were collected.
Why Leading Biology?
At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.
Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.
Working with us, you will get the guaranteed service to accommodate your requirements.
· Vigorous quality control system to ensure the required quality and reproducibility
· Competitive price with fast turnaround time
Contact Information
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