Vector Construction

Plasmid vector construction is an essential step for molecular microbiology. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Plasmids were recognized as a convenient means to package foreign DNA for molecular cloning strategies allowing for mass production of certain genes and encoding proteins in a desired host. Thus, gene cloning and vector construction are routine procedures in genomic studies.

Fig. 1 Construction of genomic vectors for gene expression

Transfection 

Co-localization Analysis

Co-localization analysis is an advanced digital imaging tool to observe the spatial overlap between two (or more) different fluorescent labels, each having a separate emission wavelength to see if the different “targets” are located in the same area of the cell or very near to one another. This technique is important to many cell biological and physiological studies during the demonstration of a relationship between pairs of bio-molecules.

Fig.2 Co-localization Analysis

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