Introduction
The Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavior of individual migrating cells can be studied by modifying the assay for video microscopy. The assay works well with freshly-isolated human neutrophils. Monocytes can also be observed to migrate but require more time.
The procedure makes use of a tissue culture dish filled with an agarose mixture. Chemoattractant diffuses from wells in the agarose to form a gradient. Cells in nearby wells can be monitored as they migrate in the direction of the chemoattractant source.
5. Detection and Analyzing.
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