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Location: Home > Custom Services > Molecular Biology Services > Soft Agar Assay

Soft Agar Assay

Date: 2018-01-25 Author: Leading Biology Click: 1086

Introduction

The soft agar assay is a well-established method used to detect cell transformation and tumorigenicity. This assay requires that cells grow in an anchorage-independent manner, a hallmark of cancer cells, but not normal cells, moreover, growth in soft agar is strongly correlated to tumorigenicity in animals. Compare to the cells grown in 2D monolayers attached to plates, such 3D growth conditions more accurately reflect the natural environment of cancer cells and are crucial to be performed before animal studies.


For this assay, cells (pretreated with carcinogens or carcinogen inhibitors) are cultured with appropriate controls in soft agar medium for 21-28 days. Following this incubation period, formed colonies can either be analyzed morphologically using cell stain and quantifying the number of colonies formed per well.


Soft Agar Assay


Procedures:
1. Preparation of base agar.
2. Adding cell agar layer onto the base agar.
3. Formation of cell colonies.
4. Agar dissolve (homogeneous cell suspension formed).

5. Cell lysed and detected.


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