MTT Assay_Molecular Biology Services_Custom Services

Introduction

MTT assay is an assay to detect cell viability which depends upon a mitochondrial dehydrogenase acting upon MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) to produce dark-blue formazan. The formazan is then solubilized, and the concentration determined by optical density at 570 nm.

MTT Assay

Tetrazolium dye reduction is dependent on NAD(P)H-dependent oxidoreductase enzymes largely in the cytosolic compartment of the cell, the reduction of MTT and other tetrazolium dyes depends on the cellular metabolic activity due to NAD(P)H flux. Cells with a low metabolism reduce very little MTT, while rapidly dividing cells exhibit high rates of MTT reduction. Thus, the MTT assay could be used for cell proliferation.

Procedures:
1. Cell culture.
2. MTT incubation.
3. Dissolve the formazan by DMSO.
4. Detection by strip reader.

5. Data analysis.

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Contact Information

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