Introduction

Fluorescence-activated cell sorting (FACS) is a derivative of flow cytometry which provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. The technique could provide fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.

During FACS, the cell suspension is entrained in the center of a narrow, rapidly flowing stream of liquid. The flow is arranged so that there is a large separation between cells relative to their diameter. A vibrating mechanism causes the stream of cells to break into individual droplets, each droplet contains one cell. An electrical charging ring is placed just at the point where the stream breaks into droplets, the charged droplets then fall through an electrostatic deflection system that diverts droplets into containers based upon their charge.

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