Introduction

Lentivirus can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. There are many advantages of using lentivirus to generate stable cell lines: unlike short-term protein expression observed using transient transfection approaches, generating cell lines using lentiviral vectors enables long-term protein expression studies, additionally, engineered lentiviral transfer vector that is embedded with a matrix-attachment region (MARs) sequence may provide position-independent transgene expression. Compare to conventional stable cell line construction, transduction with lentivirus produces a much higher positive clone rate and the target always co-exists with the selection marker, thus reduce the cost, labor, and time spent selecting stable high expression clones.

Procedures:

6. Validation of the high-expression clone by Western Blot.

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