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Location: Home > Custom Services > Molecular Biology Services > Site-directed Mutagenesis Service

Site-directed Mutagenesis Service

Date: 2018-01-24 Author: Leading Biology Click: 859

Introduction

Side-directed Mutagenesis (SDM) is an in vitro procedure that uses custom designed oligonucleotide primers to confer a specific and intentional mutation in a double-stranded plasmid DNA. The SDM is a very useful tool for gene research, it could be used to study changes in protein activity that occur as a result of the DNA manipulation; to select or screen for mutations that have a desired property, or to introduce or remove restriction endonuclease site or tags. 


Generally, we introduce site-directed mutagenesis through PCR method, including the insertion, deletion and point mutation. The procedures are like below:


Site-directed Mutagenesis Service


1. Design a primer which contains the desired change, which could be based substitution, addition or deletion.

2. Perform PCR: During PCR, the mutation is incorporated into the amplicon, replacing the original sequence.


Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.


· Vigorous quality control system to ensure the required quality and reproducibility

· Competitive price with fast turnaround time


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)



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