Introduction

Microsatellite, also called Short Tandem Repeats (STRs) or Single Sequence Repeats (SSRs), is a genetic marker that can be used to locate a specific segment of genetic material that has a known location on a chromosome. Microsatellites are codominant in nature, highly polymorphic, easily typed, and Mendelian inherited, these properties made them very suitable for the study of population structure, pedigree analysis and detect differences among closely related species. The major advantages of microsatellite markers are short size range, uninterrupted stretches of identical repeat units, the high proportion of polymorphisms, insights gained in understanding the mutational process, etc. These advantages help to develop statistical procedures for interpopulation comparisons and made micorsatellite marker be a widely used marker for high-resolution population analysis.

8. Sequencing and confirmation of microsatellites.

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