Introduction

The enzyme-linked immunosorbent assay (ELISA) is a plate-based immunological assay designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones in biological samples. There are three different types of ELISA: direct ELISA, competitive ELISA and sandwich EILSA.

Fig.1 Different types of ELISA

Among the three types, sandwich ELISA is the most widely used method for the detection of samples. Sandwich ELISA used two sets of antibodies for the product detection, the procedure is as follows:

1. The primary antibody (capture antibody) is the antibody raised against the antigen of interest, they were coated on the EILSA plate before the sample was added, any excess and unbound antibody is then washed from the plate.

2. The sample was added into the plate, any antigen found in the sample would bind to the primary antibody which already coated on the plate, again, any excess sample is washed from the plate.

3. The secondary antibody (detection antibody) was added. The secondary antibody is labeled with an enzyme and would bind to any target antigen already bound to the plate.

4. The substrate was added to the plate. The enzyme on the plate would convert the substrate into a colored product which can be measured using a plate reader.

Fig. 2 Sandwich ELISA

Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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