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Location: Home > Information Center > Technical FAQs > Protein Technology Column > What factors should I optimize to increase protein yield exp

What factors should I optimize to increase protein yield exp

Date: 2020-04-09 Author: Leading Biology Click: 553

If you are sure you can see mutant protein being expressed even at low concentration, according to me best would be to do a western blotting thats the prefect way to confirm the expressed protein. otherwise you can also cut out the band from gel and send for LC/MS-MS to detect the expressed protein. Personally if i know my protein is being expressed , I wont spend lot of time in optimisation as takes lot of time. rather than I will try to prove that protein is being expressed if thats the main aim.

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