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Location: Home > Information Center > Technical FAQs > Genome Editing Technology Column > How do I use CRISPR/Cas9?

How do I use CRISPR/Cas9?

Date: 2020-04-02 Author: Leading Biology Click: 548

CRISPR is the coolest technology in a biologist’s toolbox because of its unique blend of being extremely powerful yet relatively simple to use. The technique relies on two basic components: a Cas nuclease that cuts the DNA and a guide RNA that tells the nuclease precisely where in the genome to cut.

 

The best way to approach any challenge is to break it down into smaller steps. Broadly speaking, every CRISPR experiment can be divided into three main steps:

1. Design - Ensure that you select the optimum guide RNA and other components for your experiment

2. Edit - Introduce the CRISPR components into cells to allow the genome engineering to occur

3. Analyze - Verify the effectiveness of your experiment and move on to the next steps

 

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