Introduction

The Poly (ADP-ribose) Polymerases (PARPs) are an emerging family of enzymes that detects and signals DNA damage to repair mechanisms. It’s activated in response to single-strand DNA breaks and subsequently attaches to regions of damaged DNA. PARP then catalyzes the synthesis of poly (ADP-ribose) (PAR) chains on itself and adjacent nuclear proteins. PAR chains also serve as a signal for other DNA repair enzymes. After the DNA is repaired, the PAR chains are degraded by PAR glycohydrolase (PARG). However, the PAR generates ADP-ribose (ADPr) modifications onto target proteins using NAD+ as a substrate. Extensive DNA damage can lead to the depletion of intracellular NAD+ and energy depletion-induced necrosis.

PARP Universal Colorimetric Assay is one of the methods which could be used to analyze the PARP. This method measures PARP in cells and tissues by detecting the incorporation of biotinylated poly (ADP-ribose) (PAR) onto histone proteins in a 96-strip well microplate format, it’s useful for testing whether DNA is damaged, if damaged DNA is from non-apoptotic cells, or the effectiveness of PARP inhibitors.

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