Introduction
Cell subculture is a cell or microbiological culture method made by transferring some or all cells from a previous culture to a new vessel with a fresh growth medium to provide more room for continued growth.
Depending on the properties of the cells, there are two different ways for cell subculture:
1. For non-adherent cell culture, the cells are only grown in solution but won’t attach to the surface, these cells can be subcultured by simply taking a small volume of the primary culture and diluting it in fresh growth medium.
2. For adherent cell culture, epically the culture of many mammalian cell lines, the cells would attach to a surface such as the bottom of the culture flask. These cell types have to be detached from the surface by the trypsin before they can be subcultured. The procedures are like below:

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